کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6274029 | 1614811 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dissociation of doublecortin expression and neurogenesis in unipolar brush cells in the vestibulocerebellum and dorsal cochlear nucleus of the adult rat
ترجمه فارسی عنوان
جداسازی بیان دوکورتین و نوروژنز در سلولهای یکپارچه برس در هسته آویشن و کچلی در موشهای بالغ
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کلمات کلیدی
PBSDABDCNUBCsDcx3,3′-diaminobenzidine - 3،3'-diaminobenzidineBSA - BSAbovine serum albumin - آلبومین سرم گاوGlucose oxidase - آنزیم گلوکزاکسیدازBrdU - بروموداکسی اوریدینVestibular compensation - جبران Vestibulardoublecortin - دوچرخهunipolar brush cells - سلولهای براش یکپارچهAuditory system - سیستم شنواییPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریTransition zone - منطقه انتقالNeurogenesis - نوروژنزdorsal cochlear nucleus - هسته کچلی پشتیTinnitus - وزوز گوشPlasticity - پلاستیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
چکیده انگلیسی
We have previously shown expression of the protein doublecortin (DCX) in unipolar brush cells (UBCs) in the dorsal cochlear nucleus and vestibulocerebellum of the adult rat. We also saw DCX-immunoreactive elements with the appearance of neuroblasts around the fourth ventricle. Expression of DCX is seen in newborn and migrating neurons and hence considered a correlate of neurogenesis. There were two interpretations of the expression of DCX in UBCs. One possibility is that there might be adult neurogenesis of this cell population. Adult neurogenesis is now well-established, but only for the dentate gyrus of the hippocampus and the subventricular zone. The other possibility is that there is prolonged expression of DCX in adult UBCs that may signal a unique role in plasticity of these neurons. We tested the neurogenesis hypothesis by systemic injections of bromodeoxyuridine (BrdU), a thymidine analog, followed by immunohistochemistry to examine the numbers and locations of dividing cells. We used several different injection paradigms, varying the dose of BrdU, the number of injections and the survival time to assess the possibility of neuronal birth and migration. We saw BrdU-labeled cells in the cerebellum and brainstem; cell division in these regions was confirmed by immunohistochemistry for the protein Ki67. However, neither the numbers nor the distribution of labeled nuclei support the idea of adult neurogenesis and migration of UBCs. The function of DCX expression in UBC's in the adult remains to be understood.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 265, 18 April 2014, Pages 323-331
Journal: Neuroscience - Volume 265, 18 April 2014, Pages 323-331
نویسندگان
N. Paolone, S. Manohar, S.H. Hayes, K.M. Wong, R.J. Salvi, J.S. Baizer,