کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6274792 | 1614829 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Three distinct motifs within the C-terminus of acid-sensing ion channel 1a regulate its surface trafficking
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کلمات کلیدی
ASIC1aacid-sensing ion channel 1aPDZ binding motifCho - برایanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceChinese Hamster Ovary - تخمدان هامستر چینیendoplasmic reticulum - شبکه آندوپلاسمی Trafficking - قاچاقwild-type - نوع وحشیhemagglutinin - هماگلوتینینGlycosylation - گلیکوزیله شدن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Various protein motifs play a key role in regulating protein biogenesis and trafficking. Here, we discovered that three distinct motifs regulate the trafficking of acid-sensing ion channel 1a (ASIC1a), the primary neuronal proton receptor which plays critical roles in neurological diseases including stroke, multiple sclerosis and seizures. Mutating the PDZ binding motif of ASIC1a increased its surface expression and current density. In contrast, mutating either a RRGK motif or a KEAKR motif reduced ASIC1a surface expression and acid-activated current density. Mutating or deleting the RRGK motif also reduced pH sensitivity and the rate of desensitization of ASIC1a. These changes were likely due to a change in ASIC1a biogenesis; mutating either the RRGK or KEAKR motif reduced N-glycosylation of ASIC1a while mutating the PDZ binding motif had the opposite effect. Our results demonstrate that these C-terminal motifs are important for ASIC1a trafficking and channel function. In addition, in contrast to multiple previous studies, which all show that K/R containing motifs lead to endoplasmic reticulum (ER) retention, our findings indicate that these motifs can also be required for efficient trafficking.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 247, 5 September 2013, Pages 321-327
Journal: Neuroscience - Volume 247, 5 September 2013, Pages 321-327
نویسندگان
L. Jing, X.-P. Chu, X.-M. Zha,