Inhibition of endoplasmic reticulum Ca2+ ATPase in preBötzinger complex of neonatal rat does not affect respiratory rhythm generation

► We examined the effect of blocking SERCA on respiratory rhythm generation in an in vitro preparation. ► Thapsigargin and CPA do not affect period or amplitude of respiratory-related motor output. ► SERCA blockade and depletion of Ca2+ stores do not affect the mechanisms for rhythm generation. ► We suggest that Ca2+ from intracellular stores does not play a relevant role for rhythm generation.