Cellular and Molecular NeuroscienceResearch Paper17β-estradiol and progesterone regulate multiple progestin signaling molecules in the anteroventral periventricular nucleus, ventromedial nucleus and sexually dimorphic nucleus of the preoptic area in fema

Recent work identified novel progestin signaling molecules, including progesterone receptor membrane component 1 (Pgrmc1), Pgrmc2, serpine mRNA binding protein 1 (Serbp1), progestin and adiponectin receptors 7 (Paqr7) and Paqr8. These molecules mediate rapid progesterone (P4) effects in non-neural tissue and we recently mapped their expression in the brain. Many rapid effects of P4 require 17β-estradiol (E2) and P4 priming; therefore, we examined the effects of ovarian hormones on the expression of these non-classical progestin signaling molecules. We focused specifically on the anteroventral periventricular nucleus (AVPV), the sexually dimorphic nucleus of the preoptic area (SDN-POA) and the ventrolateral portion of the ventromedial nucleus (VMNvl). These brain nuclei are important for female reproduction. Ovariectomized adult female rats were implanted with capsules containing sesame oil or E2, and injected 48 h later with sesame oil or P4. Brains were collected 8 h later and RNA was isolated from the AVPV, SDN-POA and VMNvl. We assessed the effects of ovarian hormones on mRNA levels using quantitative polymerase chain reaction (QPCR). In the AVPV, Serbp1 mRNA levels were increased by P4 in the presence of E2, and Paqr8 was downregulated by P4 alone. In the SDN-POA, combined E2 and P4 increased Pgrmc1 and Serbp1 mRNA levels, and E2 alone increased Paqr8 mRNA levels. Finally, in the VMNvl, P4 increased mRNA levels encoding Pgrmc1, Pgrmc2 and Serbp1, and the combination of E2 and P4 increased Pgrmc1 and Serbp1 mRNA levels. Paqr7 was not regulated by E2 or P4 in any brain region examined. In summary, we showed that ovarian hormones regulate novel progestin signaling molecules in brain regions important for the neuroendocrine control of reproduction.

Research highlights▶Non-classical progesterone signaling molecules are regulated by ovarian steroids in the brain. ▶Rapid progestin signaling molecules are found in brain nuclei that regulate female reproduction. ▶Ovarian steroids regulate Pgrmc1, Pgrmc2, Serbp1 and Paqr8 in a brain nuclei-specific manner.