کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6276746 | 1295742 | 2010 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Organization and neurochemical properties of intersegmental interneurons in the lumbar enlargement of the adult rat
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSCommissural interneuronVGLUT2VGLUT1VGATGLYT2LSNGad67CpGeGFPDABHRPCTBGAD3,3′-diaminobenzidine - 3،3'-diaminobenzidineglutamic acid decarboxylase - glutamic acid dearboxylasephosphate buffer - بافر فسفاتCin - جینvesicular GABA transporter - حامل ویسکوزر GABAvesicular glutamate transporter 2 - حمل کننده گلوتامات vesicular 2vesicular glutamate transporter 1 - حمل کننده گلوتامات وزیکولار 1Neuronal network - شبکه عصبیSpinal cord - طناب نخاعیPhosphate buffered saline - فسفات بافر شورLateral spinal nucleus - هسته ستون فقرات جانبیhorseradish peroxidise - پراکسید هیدروژنCalcium binding protein - پروتئین اتصال دهنده کلسیمgreen fluorescent protein - پروتئین فلورسنت سبزNeurotransmitter - پیامرسان عصبی یا انتقال دهنده عصبی ChAT - چتCentral Pattern Generator - ژنراتور الگوی مرکزیMotor control - کنترل حرکتیcholine acetyltransferase - کولین استیل ترانسفرازglycine transporter 2 - گلیسین حمل کننده 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Organization and neurochemical properties of intersegmental interneurons in the lumbar enlargement of the adult rat Organization and neurochemical properties of intersegmental interneurons in the lumbar enlargement of the adult rat](/preview/png/6276746.png)
چکیده انگلیسی
Intersegmental interneurons with relatively short axons perform an important role in the coordination of limb movement but surprisingly little is known about their organization and how they contribute to neuronal networks in the adult rat. We undertook a series of anatomical tract-tracing studies to label cell bodies and axons of intersegmental neurons in the lumbar cord and characterized their neurochemical properties by using immunocytochemistry. The b-subunit of cholera toxin was injected into L1 or L3 segments of seven rats in the vicinity of lateral or medial motor nuclei. In L5 lumbar segments, cells were found to be concentrated in contralateral lamina VIII, and in ipsilateral lamina VII and laminae V-VI following injections into the lateral and medial motor nuclei respectively. About 25% of labelled cells contained calbindin or calretinin or a combination of both. Calbindin positive cells were mainly distributed within the ipsilateral side of the L5 segment, especially within the ipsilateral dorsal horn whereas there was a concentration of calretinin cells in contralateral lamina VIII. A small population of cells around the central canal were cholinergic. We also examined axon terminals that projected from L1/3 to the L5 contralateral lateral motor nucleus. The majority of these axons were excitatory (75%) and made direct contacts with motoneurons. However, most inhibitory axons in L5 contained a mixture of GABA and glycine (20%) and about 22% of the total population of axons contained calbindin. In contrast, 19% of all intra-segmental axons in the L3 contralateral lateral motor nucleus were found to be purely glycinergic and 17% contained a mixture of GABA and glycine. This study shows that short range interneurons form extensive ipsi- and contralateral projections within the lumbar enlargement and that many of them contain calcium binding proteins. Those projecting contralaterally to motor nuclei are predominantly excitatory.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 171, Issue 2, 1 December 2010, Pages 461-484
Journal: Neuroscience - Volume 171, Issue 2, 1 December 2010, Pages 461-484
نویسندگان
T.T. Liu, B.A. Bannatyne, D.J. Maxwell,