کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6277061 | 1295747 | 2010 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The pattern of expression of guanine nucleotide-binding protein β3 in the retina is conserved across vertebrate species
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
GAPDHRGEmGluR6Lim3TransducinGNB3OPLONLINLPRLIPLPKCRT-PCRPSD-95PNAGCLERGGPCRspeanut agglutinin - آگلوتینین بادام زمینیelectroretinogram - الکتروترینوگرامRetinopathy - رتینوپاتیbipolar cell - سلول دو قطبیPhotoreceptor - فتوتراپیouter plexiform layer - لایه بیرونی بیرونیouter nuclear layer - لایه بیرونی هسته ایinner plexiform layer - لایه داخلی لایهinner nuclear layer - لایه داخلی هسته ایganglion cell layer - لایه سلول گانگلیونیPhotoreceptor layer - لایه فتوترپتورwild-type - نوع وحشیReverse-transcriptase polymerase chain reaction - واکنش زنجیره ای پلیمراز معکوس ترانس کریتازG-proteins - پروتئین GGuanine nucleotide-binding proteins - پروتئین های اتصال دهنده نوکلئوتید گوانینpostsynaptic density protein-95 - پروتئین چگالی پستانیپتیک 95glyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Guanine nucleotide-binding protein β3 (GNB3) is an isoform of the β subunit of the heterotrimeric G protein second messenger complex that is commonly associated with transmembrane receptors. The presence of GNB3 in photoreceptors, and possibly bipolar cells, has been confirmed in murine, bovine and primate retinas [Lee RH, Lieberman BS, Yamane HK, Bok D, Fung BK (1992) J Biol Chem 267:24776-24781; Peng YW, Robishaw JD, Levine MA, Yau KW (1992) Proc Natl Acad Sci U S A 89:10882-10886; Huang L, Max M, Margolskee RF, Su H, Masland RH, Euler T (2003) J Comp Neurol 455:1-10]. Studies have indicated that a mutation in the GNB3 gene causes progressive retinopathy and globe enlargement (RGE) in chickens. The goals of this study were to (1) examine the expression pattern of GNB3 in wild-type and RGE mutant chickens, (2) characterize the types of bipolar cells that express GNB3 and (3) examine whether the expression of GNB3 in the retina is conserved across vertebrate species. We find that chickens homozygous for the RGE allele completely lack GNB3 protein. We find that the pattern of expression of GNB3 in the retina is highly conserved across vertebrate species, including teleost fish (Carassius auratus), frogs (Xenopus laevis), chickens (Gallus domesticus), mice (Mus musculata), guinea-pigs (Cavia porcellus), dogs (Canis familiaris) and non-human primates (Macaca fasicularis). Regardless of the species, we find that GNB3 is expressed by Islet1-positive cone ON-bipolar cells and by cone photoreceptors. In some vertebrates, GNB3-immunoreactivity was observed in both rod and cone photoreceptors. A protein-protein alignment of GNB3 across different vertebrates, from fish to humans, indicates a high degree (>92%) of sequence conservation. Given that analogous types of retinal neurons express GNB3 in different species, we propose that the functions and the mechanisms that regulate the expression of GNB3 are highly conserved.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 169, Issue 3, 1 September 2010, Pages 1376-1391
Journal: Neuroscience - Volume 169, Issue 3, 1 September 2010, Pages 1376-1391
نویسندگان
E.R. Ritchey, R.E. Bongini, K.A. Code, C. Zelinka, S. Petersen-Jones, A.J. Fischer,