کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6277096 | 1295748 | 2010 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Opposing functions of chondroitin sulfate and heparan sulfate during early neuronal polarization
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کلمات کلیدی
PBSHAaseUSTHeparitinaseMCSPGlcAN-acetylgalactosamineGalNAcembryonic day 16E16PFAFAKGFPBSA - BSAbovine serum albumin - آلبومین سرم گاوGlucuronic acid - اسید گلوکورونیکChase - تعقیبFocal contact - تماس کانونیnumerical aperture - دیافراگم عددیExtracellular matrix - ماتریکس خارج سلولیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHippocampal neuron - نورون هیپوکامپHeparan sulfate - هپاران سولفاتHyaluronidase - هیالورونیدازparaformaldehyde - پارافرمالدهیدProteoglycan - پروتئوگلیکانgreen fluorescent protein - پروتئین فلورسنت سبزChondroitin sulfate - کندرویتین سولفاتChondroitinase - کندرییتینازfocal adhesion kinase - کیناز چسبندگی کانونیGlycosaminoglycan - گلیکوزآمینوگلیکان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Axon-dendrite polarity of neurons is essential for information processing in the nervous system. Here we studied the functions of chondroitin sulfate (CS) and heparan sulfate (HS) in neuronal polarization using cultured dissociated hippocampal neurons. Immunohistochemical analyses of early cultured neurons indicated the distribution of these glycosaminoglycans to be quite different. While CS epitopes were accumulated in the focal contacts present in axons and cell bodies, those of HS were detected ubiquitously on the cell surface including on dendrites and axons. Treatment with chondroitinase (CHase) ABC, which degrades CS, and knockdown of a CS sulfotransferase, N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase (4,6-ST), which is involved in the biosynthesis of oversulfated structures, induced the formation of multiple axons in hippocampal neurons. Time-lapse recordings revealed the multiple axons of CHase ABC-treated neurons to be highly unstable, extending and retracting, repeatedly. CHase ABC-treatments suggested that CS is involved in the formation of phosphorylated focal adhesion kinase-positive focal contacts. Thus, CS may enhance integrin signaling in the nascent axons, supporting axon specification. On the other hand, when neurons were treated with heparitinases that specifically degrade HS, neurons with a single axon increased. The axons of HSase-treated neurons extended steadily and showed almost no retraction. These results suggest that CS stabilizes and HS destabilizes the growth of axons in an opposing manner, contributing to early neuronal polarization.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 169, Issue 4, 15 September 2010, Pages 1535-1547
Journal: Neuroscience - Volume 169, Issue 4, 15 September 2010, Pages 1535-1547
نویسندگان
K. Nishimura, M. Ishii, M. Kuraoka, K. Kamimura, N. Maeda,