کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6277359 | 1295754 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Calcium-induced calcium release contributes to synaptic release from mouse rod photoreceptors
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کلمات کلیدی
RBCOPLSerca2l-AP4CPPGl-(+)-2-amino-4-phosphonobutyric acid - L - (+) - 2-amino-4-phosphonobutyric acidCalcium-induced calcium release - انتشار کلسیم ناشی از کلسیمsynaptic transmission - انتقال سیناپسیRyanodine - رایانودینsarco/endoplasmic reticulum Ca2+-ATPase - سارکو / سلولهای اندوپلاسمی Ca2 + -ATPaseendoplasmic reticulum - شبکه آندوپلاسمی outer plexiform layer - لایه بیرونی بیرونیCICR - کمیته بینالمللی صلیب سرخ
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
We tested whether calcium-induced calcium release (CICR) contributes to synaptic release from rods in mammalian retina. Electron micrographs and immunofluorescent double labeling for the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2) and synaptic ribbon protein, ribeye, showed a close association between ER and synaptic ribbons in mouse rod terminals. Stimulating CICR with 10 μM ryanodine evoked Ca2+ increases in rod terminals from mouse retinal slices visualized using confocal microscopy with the Ca2+-sensitive dye, Fluo-4. Ryanodine also stimulated membrane depolarization of individual mouse rods. Inhibiting CICR with a high concentration of ryanodine (100 μM) reduced the electroretinogram (ERG) b-wave but not a-wave consistent with inhibition of synaptic transmission from rods. Ryanodine (100 μM) also inhibited light-evoked voltage responses of individual rod bipolar cells (RBCs) and presumptive horizontal cells recorded with perforated patch recording techniques. A presynaptic site of action for ryanodine's effects is further indicated by the finding that ryanodine (100 μM) did not alter currents evoked in voltage-clamped RBCs by puffing the mGluR6 antagonist, (RS)-α-cyclopropyl-4-phosphonophenylglycine (CPPG), onto bipolar cell dendrites in the presence of the mGluR6 agonist l-(+)-2-amino-4-phosphonobutyric acid (l-AP4). Ryanodine (100 μM) also inhibited glutamatergic outward currents in RBCs evoked by electrical stimulation of rods using electrodes placed in the outer segment layer. Together, these results indicate that, like amphibian retina, CICR contributes to synaptic release from mammalian (mouse) rods. By boosting synaptic release in darkness, CICR may improve the detection of small luminance changes by post-synaptic neurons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 165, Issue 4, 17 February 2010, Pages 1447-1456
Journal: Neuroscience - Volume 165, Issue 4, 17 February 2010, Pages 1447-1456
نویسندگان
N. Babai, C.W. Morgans, W.B. Thoreson,