کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6277753 | 1295775 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Estrogen decreases 5-HT1B autoreceptor mRNA in selective subregion of rat dorsal raphe nucleus: Inverse association between gene expression and anxiety behavior in the open field
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کلمات کلیدی
OFTselective 5-HT reuptake inhibitorRDMMVMCVM5-HT1B5-HT1AovariectomizedOVX5-HTDRNISHHtph2CDMIn situ hybridization - Hybridization در محلOpen field test - آزمایش میدان بازOvariectomy - اوفورکتومی، تخمدان برداریtryptophan hydroxylase-2 - تریپتوفان هیدروکسیلاز 2SSRI - مهارکنندههای بازجذب سروتونینdorsal raphe nucleus - هسته رافهOvarian hormones - هورمون های تخمدانtryptophan hydroxylase - هیدروکسیلاز تریپتوفانin situ hybridization histochemistry - هیستوشیمی هیبریداسیون in situ
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have recently shown that estrogen decreases anxiety and increases expression of tryptophan hydroxylase-2 (TPH2), the rate-limiting enzyme for 5-HT synthesis. However, the effects of estrogen on 5-HT release and reuptake may also affect the overall availability of 5-HT in the forebrain. Estrogen has been previously shown to have no effect on the inhibitory 5-HT 1A autoreceptor (5-HT1A) in the rat dorsal raphe nuclei (DRN); however the regulation of the inhibitory 5-HT 1B autoreceptor (5-HT1B) in the midbrain raphe by estrogen has not yet been investigated. Therefore, we examined the effects of estrogen on 5-HT1B mRNA in the rat DRN, focusing on specific subregions, and whether 5-HT1B mRNA levels correlated with TPH2 mRNA levels and with anxiety-like behavior. Ovariectomized rats were treated for 2 weeks with estrogen or placebo, exposed to the open field test, and 5-HT1A and 5-HT1B mRNA was quantified by in situ hybridization histochemistry. Estrogen had no effect on 5HT1A mRNA in any of the DRN subregions examined, confirming a previous report. In contrast, estrogen selectively decreased 5-HT1B mRNA in the mid-ventromedial subregion of the DRN, where 5-HT1B mRNA was associated with higher anxiety-like behavior and inversely correlated with TPH2 mRNA levels. These results suggest that estrogen may reduce 5-HT1B autoreceptor and increase TPH2 synthesis in a coordinated fashion, thereby increasing the capacity for 5-HT synthesis and release in distinct forebrain regions that modulate specific components of anxiety behavior.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 158, Issue 2, 23 January 2009, Pages 456-464
Journal: Neuroscience - Volume 158, Issue 2, 23 January 2009, Pages 456-464
نویسندگان
R. Hiroi, J.F. Neumaier,