کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6277773 | 1295775 | 2009 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Anosmin-1 stimulates outgrowth and branching of developing Purkinje axons
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
FGFRGSADAPIAPCFGFR1MAP24,6-diamidino-2-phenylindole - 4،6-دیامیدین-2-فنیلینولRegeneration - باززاییphosphate buffer - بافر فسفاتCho - برایanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceChinese Hamster Ovary - تخمدان هامستر چینیAxon guidance - راهنمای آکسونembryonic day - روز جنینیpostnatal day - روز پس از زایمانCerebellum - مخچهmicrotubule-associated protein 2 - پروتئین مرتبط با میکروتوبول 2fibroblast growth factor receptor - گیرنده فاکتور رشد فیبروبلاستFibroblast growth factor receptor 1 - گیرنده فاکتور رشد فیبروبلاست 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Anosmin-1 stimulates outgrowth and branching of developing Purkinje axons Anosmin-1 stimulates outgrowth and branching of developing Purkinje axons](/preview/png/6277773.png)
چکیده انگلیسی
During development, Purkinje axons elongate along precise trajectories and acquire stereotypic branching patterns to innervate targets in the deep nuclei and cerebellar cortex. These processes are accomplished through cell-intrinsic mechanisms, whose operation is regulated by environmental signaling cues. Here, we show that Anosmin-1, the protein defective in the X-linked form of Kallmann syndrome, is one among such cues. Anosmin-1, that stimulates axon elongation and branching in the olfactory system, is expressed by Purkinje cells and deep nuclear neurons of the rat cerebellum during the ontogenetic period when Purkinje axons acquire their mature pattern. These neurons also express the putative Anosmin-1 receptor, fibroblast growth factor receptor 1. Application of Anosmin-1 to dissociated cultures of embryonic (embryonic day 17, E17) or postnatal (postnatal day 0, P0) rat cerebellar cells enhances neuritic elongation and exerts a strong promoting action on the budding of collateral branches and on the extension of terminal arbors. Opposite effects are observed when neutralizing anti-Anosmin-1 antibodies are applied to the same cultures. Comparable results are obtained by administering the protein or the blocking antibodies to organotypic cultures of postnatal (P0) rat cerebellum. In P10 cerebellar slices, Anosmin-1 does not enhance the spontaneous regenerative capabilities of severed Purkinje axons, but promotes the terminal outgrowth of injured neurites into embryonic neocortical explants apposed to the axotomy site. Although Anosmin-1 is unable to change the overall intrinsic growth competence of Purkinje cells, it exerts a powerful stimulatory action on the budding and extension of collateral branches and terminal plexus, contributing to the patterning of Purkinje axons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 158, Issue 2, 23 January 2009, Pages 570-584
Journal: Neuroscience - Volume 158, Issue 2, 23 January 2009, Pages 570-584
نویسندگان
S. Gianola, F. de Castro, F. Rossi,