کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6277782 | 1295775 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Soy phytoestrogens are neuroprotective against stroke-like injury in vitro
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کلمات کلیدی
PI3KB27Hanks' buffered salt solutioncalcein acetoxymethylesterG-protein-coupled receptor 30GPR30OGDMPTPHBSSDAPIRT-PCRPBS1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine - 1-متیل 4-فنیل 1،2،3،6-تتراهیدروپیریدین17β-estradiol - 17β استرادیول4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولBSA - BSAMAPK - MAPKbovine serum albumin - آلبومین سرم گاوAkt - آکتDIV - دیوdays in vitro - روز in vitrolactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH MEK - مجاهدین خلقOxygen-glucose deprivation - محرومیت گلوکز اکسیژنPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریneurobasal medium - محیط عصبیreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenmitogen-activated protein kinase kinase - پروتئین کیناز کیناز فعال Mitogen فعالCalcein AM - کالسین AMEstrogen receptor - گیرنده استروژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Diets high in soy are neuroprotective in experimental stroke. This protective effect is hypothesized to be mediated by phytoestrogens contained in soy, because some of these compounds have neuroprotective effects in in vitro models of cell death. We tested the ability of the soy phytoestrogens genistein, daidzein, and the daidzein metabolite equol to protect embryonic rat primary cortical neurons from ischemic-like injury in vitro at doses typical of circulating concentrations in human populations (0.1-1 μM). All three phytoestrogens inhibited lactate dehydrogenase (LDH) release from cells exposed to glutamate toxicity or the calcium-ATPase inhibitor, thapsigargin. In cells exposed to hypoxia or oxygen-glucose deprivation (OGD), pretreatment with the phytoestrogens inhibited cell death in an estrogen receptor (ER) dependent manner. Although OGD results in multiple modes of cell death, examination of α-spectrin cleavage and caspase-3 activation revealed that the phytoestrogens were able to inhibit apoptotic cell death in this model. In addition, blockade of phosphoinositide 3-kinase prevented the protective effects of genistein and daidzein, and blockade of mitogen-activated protein kinase prevented genistein-dependent neuroprotection. These results suggest that pretreatment with dietary levels of soy phytoestrogens can mimic neuroprotective effects observed with estrogen and appear to use the same ER-kinase pathways to inhibit apoptotic cell death.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 158, Issue 2, 23 January 2009, Pages 602-609
Journal: Neuroscience - Volume 158, Issue 2, 23 January 2009, Pages 602-609
نویسندگان
D.A. Schreihofer, L. Redmond,