کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6278139 | 1295796 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nitric oxide modulates the firing rate of the rat supraoptic magnocellular neurons
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کلمات کلیدی
dl-AP5NOSmIPSCMNCdl-2-amino-5-phosphonovaleric acidAVPmEPSC6-Cyano-7-nitroquinoxaline-2,3-dione - 6-Cyano-7-nitroquinoxaline-2،3-dioned-NAME - D-NAMEl-NAME - L-NAMENω-nitro-l-arginine methyl ester hydrochloride - N-Nitro-L-Arginine Methyl Ester Hydrochlorideoxytocin - اکسیتوسینCNQX - سیانکیوایکسSON - فرزند پسرFiring rate - نرخ آتشNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازSupraoptic nucleus - هسته Supraopticvasopressin - وازوپرسینPatch clamp - پچ گیرHAp - گام
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In vitro, nitric oxide (NO) inhibits the firing rate of magnocellular neurosecretory cells (MNCs) of hypothalamic supraoptic and paraventricular nuclei and this effect has been attributed to GABAergic activation. However, little is known about the direct effects of NO in MNCs. We used the patch-clamp technique to verify the effect of l-arginine, a precursor for NO synthesis, and NÏ-nitro-l-arginine methyl ester hydrochloride (l-NAME), an inhibitor of NOS, on spontaneous electrical activity of MNCs after glutamatergic and GABAergic blockade in Wistar rat brain slices. 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX) (10 μM) and dl-2-amino-5-phosphonovaleric acid (dl-AP5) (30 μM) were used to block postsynaptic glutamatergic currents, and picrotoxin (30 μM) and saclofen (30 μM) to block ionotropic and metabotropic postsynaptic GABAergic currents. Under these conditions, 500 μM l-arginine decreased the firing rate from 3.7±0.6 Hz to 1.3±0.3 Hz. Conversely, 100 μM l-NAME increased the firing rate from 3.0±0.3 Hz to 5.8±0.4 Hz. All points histogram analysis showed changes in resting potential from â58.1±0.8 mV to â62.2±1.1 mV in the presence of l-arginine and from â59.8±0.7 mV to â56.9±0.8 mV by l-NAME. Despite the nitrergic modulator effect on firing rate, some MNCs had no significant changes in their resting potential. In those neurons, hyperpolarizing after-potential (HAP) amplitude increased from 12.4±1.2 mV to 16.8±0.7 mV by l-arginine, but without significant changes by l-NAME treatment. To our knowledge, this is the first demonstration that NO can inhibit MNCs independent of GABAergic inputs. Further, our results point to HAP as a potential site for nitrergic modulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 155, Issue 2, 13 August 2008, Pages 359-365
Journal: Neuroscience - Volume 155, Issue 2, 13 August 2008, Pages 359-365
نویسندگان
R.R. Ventura, J.F. Aguiar, J. Antunes-Rodrigues, W.A. Varanda,