کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6279037 | 1615066 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transplantation of bone marrow mononuclear cells prolongs survival, delays disease onset and progression and mitigates neuronal loss in pre-symptomatic, but not symptomatic ALS mice
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کلمات کلیدی
eGFPMSCBMMCsSOD1 miceTNFGLT1 - GLT-1SAL - WILLamyotrophic lateral sclerosis - اسکلروز جانبی آمیوتروفیکALS - بیماری اسکلروز جانبی آمیوتروفیکCell therapy - سلول درمانیMesenchymal stem cells - سلول های بنیادی مزانشیمیBone marrow mononuclear cells - سلول های تک هسته ای مغز استخوانenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استChAT - چتcholine acetyltransferase - کولین استیل ترانسفرازglutamate transporter 1 - گلوتامات 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Cell-based therapy provides a novel strategy to restore lost neurons or modulate the degenerating microenvironment in amyotrophic lateral sclerosis (ALS). This study verified the therapeutic potential of bone marrow mononuclear cells (BMMCs) in SOD1G93A mice. BMMCs were obtained from enhanced green fluorescent protein (EGFP) transgenic C57BL/6 mice (EGFPBMMCs) or from SOD1G93A transgenic mice (mSOD1BMMCs) and given to mice at the pre-symptomatic or late symptomatic stage. Survival, body weight and motor performance data were recorded. DNA integrity was evaluated using the alkaline comet assay. The spinal cords were collected to assess motoneuron preservation and cell migration. EGFPBMMCs and mSOD1BMMCs transplantation to pre-symptomatic SOD1G93A mice prolonged survival and delayed disease progression. The effects were more significant for the EGFPBMMC-transplanted mice. In late symptomatic mice, EGFPBMMCs promoted a discrete increase in survival, without other clinical improvements. DNA from EGFPBMMCs and mSOD1BMMCs was found in the spinal cords of transplanted animals. DNA damage was not modified by BMMCs in any of the studied groups. Despite positive behavioral effects observed in our study, the limited results we observed for late transplanted mice call for caution before clinical application of BMMCs in ALS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 633, 28 October 2016, Pages 182-188
Journal: Neuroscience Letters - Volume 633, 28 October 2016, Pages 182-188
نویسندگان
Gianina Teribele Venturin, Samuel Greggio, Gabriele Zanirati, Daniel Rodrigo Marinowic, Iuri Marques de Oliveira, João Antonio Pêgas Henriques, Jaderson Costa M.D., Ph.D,