کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6279415 | 1615071 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activated caspase-9 immunoreactivity in glial and neuronal cytoplasmic inclusions in multiple system atrophy
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کلمات کلیدی
PBSNCIGCIMSADLBMSA-CMSA-pMultiple system atrophy - آتروفی سیستم چندگانهApoptosome - آپوپتوزومParkinson’s disease - بیماری پارکینسونGlial cytoplasmic inclusions - درگیری های سیتوپلاسمی گلیالDLB, Dementia with Lewy bodies - زوال عقل با اجسام لوییPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریCaspase-9 - کاسپاز-9Glial cytoplasmic inclusion - گنجاندن سیتوپلاسمیک گلیال
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Activated caspase-9 immunoreactivity in glial and neuronal cytoplasmic inclusions in multiple system atrophy Activated caspase-9 immunoreactivity in glial and neuronal cytoplasmic inclusions in multiple system atrophy](/preview/png/6279415.png)
چکیده انگلیسی
The mitochondria play an important role in apoptotic cell death, and the released cytochrome c from the mitochondria promotes the formation of the apoptosome, which contains cytochrome c, Apaf-1 and caspase-9, resulting in the activation of caspase-9 and the promotion of the apoptotic cascade. To investigate the role of mitochondria-dependent apoptotic cell death in patients with multiple system atrophy (MSA), we performed immunohistochemical studies on apoptosome-related proteins in formalin-fixed, paraffin-embedded sections from 8 normal subjects and 10 patients with MSA. We then performed double-labeling immunohistochemistry for activated caspase-9 and α-synuclein in some sections from 10 patients with MSA. In the brains with MSA, glial cytoplasmic inclusions (GCIs) and neuronal cytoplasmic inclusions (NCIs) were intensely immunoreactive for cytochrome c, Apaf-1 and caspase-9. Activated caspase-9 immunoreactivities were also confirmed to be densely localized to both GCIs and NCIs using two types of anti-cleaved caspase-9 antibodies. The semiquantitative analyses using the upper pontine sections double-immunostained with cleaved caspase-9 and α-synuclein demonstrated that approximately 80% of GCIs and NCIs were immunopositive for cleaved caspase-9. Our results suggest that the formation of the apoptosome accompanied by the activation of caspase-9 may occur in brains affected by MSA, and that a mitochondria-dependent apoptotic pathway may be partially associated with the pathogenesis of MSA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 628, 15 August 2016, Pages 207-212
Journal: Neuroscience Letters - Volume 628, 15 August 2016, Pages 207-212
نویسندگان
Yasuhiro Kawamoto, Takashi Ayaki, Makoto Urushitani, Hidefumi Ito, Ryosuke Takahashi,