کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6282171 | 1615133 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Embryonic development of GABAergic signaling in the mouse spinal trigeminal nucleus interpolaris
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کلمات کلیدی
Spinal trigeminal nucleus interpolarisSpViVGATNKCC1GADABCKCC2Cl− - Cl-glutamic acid decarboxylase - glutamic acid dearboxylaseγ-amino butyric acid - γ-آمینو اسید بوتیریکGlutamic acid decarboxylase (GAD) - اسید گلوتامیک دکربوکسیلاز (GAD)phosphate buffer - بافر فسفاتvesicular GABA transporter - حامل ویسکوزر GABACNS - دستگاه عصبی مرکزیembryonic day - روز جنینیpostnatal day - روز پس از زایمانcentral nervous system - سیستم عصبی مرکزیGABAergic synapse - سیناپس GABAergicavidin–biotin–peroxidase complex - مجتمع آویدین-بیوتین پراکسیدازGABA - گاباChloride ion - یون کلرید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the mature central nervous system, γ-amino butyric acid (GABA) is an inhibitory neurotransmitter, whereas during development, GABA induces depolarization. To examine the embryonic development of GABAergic transmission in the mouse spinal trigeminal nucleus interpolaris (SpVi), which receives sensory input from the face and is important in survival of rodents, we performed immunohistochemistry for three related molecules: glutamic acid decarboxylase (GAD), a marker of GABAergic neurons; vesicular GABA transporter (VGAT), a marker of GABAergic and glycinergic vesicles; and potassium chloride co-transporter 2 (KCC2), which shifts GABA action from excitatory to inhibitory. GAD-positive longitudinal projection fibers, where VGAT-positive dots were localized, were clearly discernible until embryonic day (E)17, and were markedly decreased in number on postnatal day 0. GAD-positive neurons were detected after E15, and GAD- and VGAT-positive axon varicosities were observed after E17. KCC2 immunolabeling was first localized in the dendrites and cell bodies of several neurons in the lateral part of the SpVi on E13 and throughout the nucleus on E17. These results suggest that the SpVi may first receive GABAergic projection fibers from extra-nuclear area before birth, and GABAergic interneurons may form synapses within the SpVi after E17. In addition, GABA action may gradually shift from excitatory to inhibitory between E13 and E17.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 566, 30 April 2014, Pages 221-225
Journal: Neuroscience Letters - Volume 566, 30 April 2014, Pages 221-225
نویسندگان
Hidemichi Kin, Jeongtae Kim, Chigusa Shimizu-Okabe, Akihito Okabe, Chitoshi Takayama,