کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6282480 | 1615141 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Trophic factor and hormonal regulation of neurite outgrowth in sensory neuron-like 50B11 cells
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کلمات کلیدی
DRGangiotensin II receptor type 2AT2NGFPGP9.5GDNFANGIIdorsal root ganglion - گانگلیون ریشه پشتیAngiotensin II - آنژیوتانسین دوaxon - آکسون Immunoreactivity - ایمنی فعالOutgrowth - رشدhigh throughput screening - غربالگری بالاnerve growth factor - فاکتور رشد عصبGlial cell line derived neurotrophic factor - فاکتور نوروترفیک مشتق از گلبول های قرمزNTFs, Neurotrophic factors - فاکتورهای رشد عصبیHormones - هورمونprotein gene product 9.5 - ژن پروتئین محصول 9.5Estrogen receptor - گیرنده استروژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Trophic factor and hormonal regulation of neurite outgrowth in sensory neuron-like 50B11 cells Trophic factor and hormonal regulation of neurite outgrowth in sensory neuron-like 50B11 cells](/preview/png/6282480.png)
چکیده انگلیسی
Sensory axon integrity and regenerative capacity are important considerations in understanding neuropathological conditions characterized by hyper- or insensitivity. However, our knowledge of mechanisms regulating axon outgrowth are limited by an absence of suitable high-throughput assay systems. The 50B11 cell line generated from rat embryonic dorsal root ganglion neurons offers a promising model for screening assays. Prior characterization shows that these cells express cytoskeletal proteins and genes encoding ion channels and neurotrophin receptors in common with sensory nociceptor neurons. In the present study we further characterized 50B11 cells in regard to their phenotypes and responsiveness to neurotrophic and hormonal factors. 50B11 cells express neuronal cytoplasmic proteins including beta-3 tubulin, peripherin (a marker of unmyelinated neurons), and the pan-neuronal ubiquitin hydrolase, PGP9.5. Only PGP9.5 immunoreactivity was uniformly distributed throughout soma and axons, and therefore presents the best means for visualizing the entire axon arbor. All cells co-express both NGF and GDNF receptors and addition of ligands increased neurite length. 50B11 cells also showed immunoreactivity for the estrogen receptor-α and the angiotensin receptor type II, and both 17-β estradiol and angiotensin II increased outgrowth by differentiated cells. 50B11 cells therefore show features reported previously for primary unmyelinated nociceptor neurons, including responsiveness to classical neurotrophins and hormonal modulators. Coupled with their ease of culture and predictable differentiation, 50B11 cells represent a promising cell line on which to base assays that more clearly reveal mechanisms regulating axon outgrowth and integrity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 558, 13 January 2014, Pages 120-125
Journal: Neuroscience Letters - Volume 558, 13 January 2014, Pages 120-125
نویسندگان
Aritra Bhattacherjee, Zhaohui Liao, Peter G. Smith,