25-Hydroxycholesterol regulates cholesterol homeostasis in the murine CATH.a neuronal cell line

► Peripheral LPS induces cholesterol 25-hydroxylase expression in mouse brain. ► 25-Hydroxycholesterol upregulates LXR target genes in a mouse neuronal cell line. ► 25-Hydroxycholesterol downregulates neuronal SREBP2-dependent transcription. ► 25-Hydroxycholesterol attenuates neuronal cholesterol biosynthesis.