Identification of dynamin as a septin-binding protein

► Septin from lipid raft of rat brain contained several isoforms forming heterooligomers. ► Dynamin was shown to be co-fractionated with septin from lipid raft. ► Dynamin fraction prepared from rat brain contained several septin isoforms. ► Bacterially expressed septin5 and septin11 bound dynamin, but septin9 did not. ► Partial co-localization of septin5 and dynamin was observed in cultured neurons.