Addition of NMDA-receptor antagonist MK801 during oxygen/glucose deprivation moderately attenuates the upregulation of glucose uptake after subsequent reoxygenation in brain endothelial cells

► Glucose transporter glut1 is up-regulated by 6 h hypoxia 15.4-fold and by oxygen/glucose deprivation (OGD) 68.7-fold. ► Blood-brain barrier mouse cell line cerebEND expresses NMDA-receptor subunits NR1, NR2A, NR2B, NR2C, NR3A and NR3B. ► NMDA-receptor subunit NR1 is significantly up-regulated by 6 h OGD 2.9-fold. ► Glucose transporters glut1 as well as sglt1 contribute to the up-regulated glucose uptake after OGD. ► Addition of 10 μM MK801 during OGD attenuates glucose uptake after further 20 h reoxygenation significantly.