Diverse polyubiquitin chains accumulate following 26S proteasomal dysfunction in mammalian neurones

▶ An extensive repertoire of non-Lys63-linked polyubiquitin chains function in 26S proteasomal targeting in mammalian neurones. ▶ Mass spectrometry approaches can be used to investigate ubiquitin-ubiquitin isopeptide linkages in their physiological context in vivo.