کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6285856 1296908 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of gonadal steroid hormones on GIRK2 gene transcription in the rat central nervous system
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Effects of gonadal steroid hormones on GIRK2 gene transcription in the rat central nervous system
چکیده انگلیسی
GIRK2 is the primary post-synaptic effector of opioids in the CNS that may contribute to the sex differences or age dependency in opioid analgesia. In the present study, we investigated the differential developmental and hormonal regulation of GIRK2 gene transcription in rats. Male and female Wistar rats were divided into infant or adult intact, gonadectomized, sham and gonadectomy + testosterone groups. Semi-quantitative RT-PCR was used to determine the levels of GIRK2 gene expression in spinal cord and brain. Our results showed that in male spinal cord/brain, the gene transcription of the infant group did not differ from expression levels of this gene in the gonadectomized or adult intact groups. Castration of males significantly decreased the expression levels of the GIRK2 gene. Testosterone replacement in castrated rats did not raise GIRK2 gene expression completely to the level of shams. In females however, a greater expression of GIRK2 gene expression was found in adult intact or gonadectomized rats than in the infant groups. Ovariectomy failed to alter GIRK2 mRNA levels significantly. No significant sex differences were observed in GIRK2 gene transcription between intact, sham and infant groups, but gonadectomy produced sex differences in GIRK2 gene transcription. The results strongly demonstrate the differential developmental and hormonal regulation of GIRK2 in the rat CNS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 431, Issue 3, 6 February 2008, Pages 201-205
نویسندگان
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