کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6287626 1615617 2012 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperUptake of gentamicin by vestibular efferent neurons and superior olivary complex after transtympanic administration in guinea pigs
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی سیستم های حسی
پیش نمایش صفحه اول مقاله
Research paperUptake of gentamicin by vestibular efferent neurons and superior olivary complex after transtympanic administration in guinea pigs
چکیده انگلیسی

Transtympanic administration of gentamicin is a widely accepted and effective approach for treating patients with intractable vertigo. Previous studies have demonstrated the uptake, distribution and effects of gentamicin in peripheral vestibular and cochlear structures after transtympanic injection. However, little is known about whether transtympanically administered gentamicin is trafficked into more central auditory and vestibular structures and its effect on these structures. In this study, we used immunofluorescence to determine the distribution of gentamicin within the auditory and vestibular brainstem. We observed gentamicin immunolabeling bilaterally in the vestibular efferent neurons, and in the superior olivary complex, and ipsilaterally in the cochlear nucleus 24 h after transtympanic administration of gentamicin, and that the drug could still be detected in these locations 30 days after injection. In contrast, no gentamicin labeling was detected in the vestibular nuclear complex. In the vestibular efferent neurons and superior olivary complex, gentamicin labeling was detected in the cytoplasm and cell processes, while in the cochlear nucleus gentamicin is mainly localized outside and adjacent to the cell bodies of neurons. Nerve fibers in cochlear nucleus, root of eighth nerve, as well as descending pathways from the superior olivary complex, are also immunolabeled with gentamicin continuously. Based on these data, we hypothesize that retrograde axonal transport of gentamicin is responsible for the distribution of gentamicin in these efferent nuclei including vestibular efferent neurons and superior olivary complex and anterograde axonal transport into the ipsilateral cochlear nucleus.

► Gentamicin was observed in vestibular efferent neurons after local administration. ► Gentamicin was observed in auditory nuclei after transtympanic administration. ► Gentamicin was detected in those locations up to 30 days after injection. ► Gentamicin traffics to these nuclei via retrograde or anterograde axonal transport.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Hearing Research - Volume 283, Issues 1–2, January 2012, Pages 169-179
نویسندگان
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