کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6288058 | 1300428 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Riemerella anatipestifer lacks luxS, but can uptake exogenous autoinducer-2 to regulate biofilm formation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Riemerella anatipestifer lacks luxS, but can uptake exogenous autoinducer-2 to regulate biofilm formation Riemerella anatipestifer lacks luxS, but can uptake exogenous autoinducer-2 to regulate biofilm formation](/preview/png/6288058.png)
چکیده انگلیسی
Riemerella anatipestifer (RA) causes major economic losses to the duck industry. Autoinducer-2 (AI-2) is a quorum-sensing signal that regulates bacterial physiology. The luxS and pfs genes are required for AI-2 synthesis in many bacterial species. pfs encodes Pfs, which functions upstream of LuxS in the biosynthesis of AI-2. In this study, we investigated the AI-2 activity of RA using an AI-2 bioassay, which showed that RA does not produce AI-2. Bioinformatic analysis indicated that the RA genome has a pfs, but not a luxS, homologue. To investigate the function of RA pfs, an avian pathogenic Escherichia coli (APEC) pfs mutant was constructed, which was subsequently transformed with a recombinant plasmid carrying RA pfs. An AI-2 bioassay demonstrated that RA pfs restored AI-2 production to the APEC pfs mutant, suggesting that RA pfs functions in AI-2 synthesis. Furthermore, we found that RA utilizes exogenous AI-2 to regulate biofilm formation. RA biofilm formation decreased significantly upon addition of exogenous AI-2. Real-time quantitative PCR results showed that expression of 13 genes related to RA biofilm formation decreased significantly when exogenous AI-2 was added to the RA culture media. These findings will benefit future studies on AI-2 regulation in RA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 166, Issue 6, JulyâAugust 2015, Pages 486-493
Journal: Research in Microbiology - Volume 166, Issue 6, JulyâAugust 2015, Pages 486-493
نویسندگان
Xiangan Han, Lei Liu, Guobo Fan, Yuxi Zhang, Da Xu, Jiakun Zuo, Shaohui Wang, Xiaolan Wang, Mingxing Tian, Chan Ding, Shengqing Yu,