کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6288524 | 1616254 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genetic characterization of strains of Saccharomyces uvarum from New Zealand wineries
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کلمات کلیدی
ITSSGMSaccharomyces uvarumYPdInternal transcribed spacer - Spacer رونویسی داخلیEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدGenome sequence - توالی ژنومyeast extract peptone dextrose - عصاره مخمر پپتون دگزستروزpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازMicrosatellite polymorphism - پلی مورفیسم میکروس تابلیت
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Genetic characterization of strains of Saccharomyces uvarum from New Zealand wineries Genetic characterization of strains of Saccharomyces uvarum from New Zealand wineries](/preview/png/6288524.png)
چکیده انگلیسی
We present a genetic characterization of 65 isolates of Saccharomyces uvarum isolated from wineries in New Zealand, along with the complete nucleotide sequence of a single sulfite-tolerant isolate. The genome of the New Zealand isolate averaged 99.85% nucleotide identity to CBS7001, the previously sequenced strain of S. uvarum. However, three genomic segments (37-87 kb) showed 10% nucleotide divergence from CBS7001 but 99% identity to Saccharomyces eubayanus. We conclude that these three segments appear to have been introgressed from that species. The nucleotide sequence of the internal transcribed spacer (ITS) region from other New Zealand isolates were also very similar to that of CBS7001, and hybrids showed complete genetic compatibility for some strains, with tetrads giving four viable progeny that showed 2:2 segregations of marker genes. Some strains showed high tolerance to sulfite, with genetic analysis indicating linkage of this trait to the transcription factor FZF1, but not to SSU1, the sulfite efflux pump that it regulates in order to confer sulfite tolerance in Saccharomyces cerevisiae. The fermentation characteristics of selected strains of S. uvarum showed exceptionally good cold fermentation characteristics, superior to the best commercially available strains of S. cerevisiae.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 46, April 2015, Pages 92-99
Journal: Food Microbiology - Volume 46, April 2015, Pages 92-99
نویسندگان
Hanyao Zhang, Keith D. Richards, Sandra Wilson, Soon A. Lee, Hester Sheehan, Miguel Roncoroni, Richard C. Gardner,