کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6289816 | 1616568 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Level 2 validation of a flow cytometric method for detection of Escherichia coli O157:H7 in raw spinach
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Level 2 validation of a flow cytometric method for detection of Escherichia coli O157:H7 in raw spinach Level 2 validation of a flow cytometric method for detection of Escherichia coli O157:H7 in raw spinach](/preview/png/6289816.png)
چکیده انگلیسی
The Bacteriological Analytical Manual (BAM) method currently used by the United States Food and Drug Administration (FDA) to detect Escherichia coli O157:H7 in spinach was systematically compared to a new flow cytometry based method. This Food and Drug Administration (FDA) level 2 external laboratory validation study was designed to determine the latter method's sensitivity and speed for analysis of this pathogen in raw spinach. Detection of target cell inoculations with a low cell count is critical, since enterohemorrhagic strains of E. coli require an infective dose of as few as 10 cells (Schmid-Hempel and Frank, 2007). Although, according to the FDA, the infectious dose is unknown (Food and Drug Administration, 1993). Therefore, the inoculation level into the spinach, a total of 2.0 ± 2.6 viable E. coli O157 cells, was specified to yield between 25% and 75% detection by the new method, out of 20 samples (10 positives and 10 negatives). This criterion was met in that the new method detected 60% of the nominally positive samples; the corresponding sensitivity of the reference method was 50%. For both methods the most likely explanation for false negatives was that no viable cells were actually introduced into the sample. In this validation study, the flow cytometry method was equal to the BAM in sensitivity and far superior in speed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 215, 23 December 2015, Pages 1-6
Journal: International Journal of Food Microbiology - Volume 215, 23 December 2015, Pages 1-6
نویسندگان
Anna J. Williams, Willie M. Cooper, Christine V. Summage-West, Lillie M. Sims, Robert Woodruff, Jessica Christman, Ted J. Moskal, Shawn Ramsaroop, John B. Sutherland, Pierre Alusta, Jon G. Wilkes, Dan A. Buzatu,