Development of a multiplex PCR assay for simultaneous detection of Theileria annulata, Babesia bovis and Anaplasma marginale in cattle

► Novel multiplex PCR for Theileria annulata, Babesia bovis and Anaplasma marginale. ► Specific and sensitive tool which can be applied to epidemiological studies. ► Simple and efficient assay which has been validated using field samples.