Molecular characterization of Leishmania infection in sand flies from Al-madinah Al-munawarah province, western Saudi Arabia

► Sand fly sampling was carried out from Al-madinah Al-munawarah. ► P. papatasi (1673 male and 1057 female), P. sergenti (109 male and 71 female). ► DNA band 650bp for L. major and 720bp for L. tropica. ► ITS1-PCR-RFLP = 200bp+57bp for L. tropica; 203bp+132bp specific for L. major.