Cell death features induced in Leishmania major by 1,3,4-thiadiazole derivatives

► Thiadiazole analogs induced loss of plasma membrane integrity, DNA breakage and proteolysis of PARP. ► These analogs were shown to induce necrosis on Leishmania major promastigotes. ► These analogs decreased acid phosphatase activity significantly during virulent growth stage of promastigotes.