کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6291502 1302492 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mitochondrial damage contribute to epigallocatechin-3-gallate induced death in Leishmania amazonensis
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Mitochondrial damage contribute to epigallocatechin-3-gallate induced death in Leishmania amazonensis
چکیده انگلیسی

Epigallocatechin-3-gallate (EGCG), the most abundant flavonoid in green tea, has been reported to have antiproliferative effects on Trypanosoma cruzi however, the mechanism of protozoan action of EGCG has not been studied. In the present study, we demonstrate the mechanism for the antileishmanial activity of EGCG against Leishmania amazonensis promastigotes. Incubation with EGCG significantly inhibited L. amazonensis promastigote proliferation in a time- and dose-dependent manner. The IC50 for EGCG at 120 h was 0.063 mM. Ultrastructural alterations of the mitochondria were observed in promastigote treated with EGCG, being the organelle injury reinforced by the decrease in rhodamine 123 fluorescence. The effects of several drugs that interfere directly with mitochondrial physiology in parasites such as Leishmania have been described. The unique mitochondrial features of Leishmania make this organelle an ideal drug target while minimizing toxicity. These data suggest mitochondrial collapse as a part of the EGCG mechanism of action and demonstrate the leishmanicidal effect of EGCG.

Highlights► EGCG induced mitochondrial collapse in Leishmania amazonensis promastigotes. ► EGCG inhibited the cellular proliferation in a time and dose-dependent manner. ► EGCG-treated promastigotes showed ultrastructural alteration of the mitochondria.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 132, Issue 2, October 2012, Pages 151-155
نویسندگان
, , , ,