Alginate microspheres encapsulated with autoclaved Leishmania major (ALM) and CpG-ODN induced partial protection and enhanced immune response against murine model of leishmaniasis

► ALM encapsulated in alginate microspheres elicit CMI and protect mice against Leishmania major infection. ► Administration of ALM with CpG ODNs elicit CMI and protect mice against L. major infection. ► ALM co-encapsulated with CpG ODNs in alginate microspheres elicit strong CMI and protect mice against L. major infection.