Selection and identification of a new adhesion protein of Cryptosporidium parvum from a cDNA library by ribosome display

► A cDNA library of the sporozoite and oocyst stages of Cryptosporidium parvum was constructed and expressed on ribosome. ► The ribosome library were bound to IECs and enriched using a multi-step panning procedure. ► A new surface adherence protein of C. parvum was selected (Cp20). ► The Cp20 which was on the oocyst and apical region of sporozoite surface has been examined. ► The Cp20 gene could be served as candidate gene of a vaccine.