کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6292000 | 1617029 | 2012 | 4 صفحه PDF | دانلود رایگان |
Adenylate kinases are key enzymes involved in cell energy management. Trypanosomatid organisms have the largest number of isoforms found in a single cell, constituting a major difference with the mammalian hosts. In this work we study an adenylate kinase, TcADK3, the only Trypanosoma cruzi protein harboring the putative peroxisomal (glycosomal) targeting signal, “-CKL”. Parasites expressing GFP fused to TcADK3 showed a strong fluorescence in the glycosomes. The same result was obtained when the tripeptide “-CKL” was added at the C-terminus of the GFP, demonstrating that this signal is necessary and sufficient for targeting proteins to glycosomes. When this tripeptide was removed from the GFP-TcADK3 fusion protein, the fluorescence was re-localized in the cytoplasm. The CKL signal could be used for targeting foreign proteins to the glycosomes. This model also provides a useful tool to study glycosomes dynamics, morphology or number in living parasites in any stage of the life cycle.
Highlights⺠This is the first report of a Trypanosoma cruzi glycosomal adenylate kinase. ⺠This adenylate kinase has a peroxisomal targeting signal. ⺠The model constitutes a tool for directing foreign molecules to glycosomes. ⺠The model is also useful for the study of glycosomes dynamics in living parasites.
Journal: Experimental Parasitology - Volume 130, Issue 4, April 2012, Pages 408-411