کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6292262 1302522 2010 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Trypanosoma brucei: Trypanosome-specific endoplasmic reticulum proteins involved in variant surface glycoprotein expression
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Trypanosoma brucei: Trypanosome-specific endoplasmic reticulum proteins involved in variant surface glycoprotein expression
چکیده انگلیسی

In Trypanosoma brucei the GPI-anchored variant surface glycoprotein (VSG) represents ∼90% of cell surface protein and a major proportion of endoplasmic reticulum (ER) biosynthetic output. We identified four trypanosomatid-specific genes encoding candidate ER-resident proteins; all were required for normal proliferation. For Tb11.01.2640 and Tb11.01.8120, an increase in VSG abundance was found on silencing, while the protein products localized to the ER; we designated these ERAP32 and ERAP18 for ER-associated protein of 32 kDa and 18 kDa. Silencing ERAP32 or ERAP18 did not alter expression levels of ISG65 or ISG75, the major surface trans-membrane domain proteins. Surface biotinylation or immunoflorescence did not identify intracellular VSG accumulation, while FACS and fluorescence microscopy indicated that the cells were not increased in size, arguing for increased VSG density on the cell surface. Therefore, ERAP32 and ERAP18 are trypanosome-specific ER-localized proteins with a major role in VSG protein export and, contrary to current paradigms, VSG is not saturated on the cell surface.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 125, Issue 3, July 2010, Pages 208-221
نویسندگان
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