Trypanosoma brucei s.l.: Microsatellite markers revealed high level of multiple genotypes in the mid-guts of wild tsetse flies of the Fontem sleeping sickness focus of Cameroon

► This study reveals multiple-genotypes of T. brucei in the mid-guts of wild tsetse. ► It highlights a wide range of T. brucei genotypes circulating in tsetse mid-guts. ► Multiple genotypes involving T. b. gambiense are 2.2 time the single infections. ► Therefore, multiple infections are frequent in the mid-guts of wild tsetse flies. ► They involve various T. b. gambiense strains and different trypanosome species.