کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6312150 1618961 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetic response of a genotoxicity biomarker in the three-spined stickleback and implication for environmental monitoring
ترجمه فارسی عنوان
پاسخ سینتیکی نشانگر زیستی ژنوتوکسیک در سه ستون فقرات و کاربرد آن برای نظارت بر محیط زیست
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
چکیده انگلیسی


- DNA damages were quantified in stickleback erythrocytes exposed to MMS.
- A fast dose response relationship was observed during 12 day exposure period.
- Low observed depuration explained by erythrocyte turn-over.
- DNA damage measurement in stickleback can be considered as an ideal biomarker.

The ultimate sink for the majority of anthropogenic compounds are the aquatic ecosystems, either through direct discharges or indirectly through hydrologic or atmospheric processes, possibly leading to long-term adverse effects in aquatic living resources. In order to assess exposure, fate and effects of chemical contaminants, aquatic ecotoxicologists have developed a large array of early-warning biomarkers proving that toxicants have entered organisms, have been distributed between organs and have triggered toxic effects regarding critical targets. However, optimal use of biomarkers in environmental studies previously requires in-depth knowledge of the kinetics of response of biomarkers. This work aimed to define as a first step of a validation process the kinetic response of a genotoxicity biomarker recently developed in the three-spined stickleback (Gasterosteus aculeatus). DNA damage was assessed in stickleback erythrocytes after in vivo exposure for 12 days to methylmethanesulfonate (MMS), an alkylating compound, followed by a 20 day-recovery period. Results show a dose-response relationship, time to maximal induction being reached after 6 days at the highest MMS concentration. No acclimation process was noticed during exposure whatever the MMS concentration, and genotoxicity decreased during the recovery phase only in fish exposed to the highest MMS concentration, suggesting more an effect of erythrocyte turn-over than of DNA repair system on the observed DNA damage level. Further field experiments are needed before including this genotoxicity biomarker in a battery of biochemical markers to monitor adverse effects of pollutants on fish health.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 102, April 2014, Pages 6-11
نویسندگان
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