کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6315893 | 1619157 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Kinetics of nutrient enhanced crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2 isolated from Guwahati refinery, India
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Bacterial degradation of crude oil in response to nutrient treatments has been vastly studied. But there is a paucity of information on kinetic parameters of crude oil degradation. Here we report the nutrient stimulated kinetic parameters of crude oil degradation assessed in terms of CO2 production and oil removal by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2. The hydrocarbon degradation rate of P. aeruginosa AKS1 in oil only amended sediment was 10.75 ± 0.65 μg CO2-C gâ1 sediment dayâ1 which was similar to degradation rate in sediments with no oil. In presence of both inorganic N & P, the degradation rate increased to 47.22 ± 1.32 μg CO2-C gâ1 sediment dayâ1. The half-saturation constant (Ks) and maximum degradation rate (Vmax) for P. aeruginosa AKS1 under increasing N and saturating P concentration were 13.57 ± 0.53 μg N gâ1 sediment and 39.36 ± 1.42 μg CO2-C gâ1 sediment dayâ1 respectively. The corresponding values at increasing P and a constant N concentration were 1.60 ± 0.13 μg P gâ1 sediment and 43.90 ± 1.03 μg CO2-C gâ1 sediment dayâ1 respectively. Similarly the degradation rate of Bacillus sp. AKS2 in sediments amended with both inorganic nutrients N & P was seven fold higher than the rates in oil only or nutrient only treated sediments. The Ks and Vmax estimates of Bacillus sp. AKS2 under increasing N and saturating P concentration were 9.96 ± 1.25 μg N gâ1 sediment and 59.96 ± 7.56 μg CO2-C gâ1 sediment dayâ1 respectively. The corresponding values for P at saturating N concentration were 0.46 ± 0.24 μg P gâ1 sediment and 63.63 ± 3.54 μg CO2-C gâ1 sediment dayâ1 respectively. The rates of CO2 production by both isolates were further stimulated when oil concentration was increased above 12.5 mg gâ1 sediment. However, oil degradation activity declined at oil concentration above 40 mg gâ1 sediment when treated with constant nutrient: oil ratio. Both isolates exhibited alkane hydroxylase activity but aromatic degrading catechol 1, 2-dioxygenase and catechol 2, 3-dioxygenase activities were shown by P. aeruginosa AKS1 only.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Pollution - Volume 216, September 2016, Pages 548-558
Journal: Environmental Pollution - Volume 216, September 2016, Pages 548-558
نویسندگان
Bobby Chettri, Arghya Mukherjee, James S. Langpoklakpam, Dhrubajyoti Chattopadhyay, Arvind K. Singh,