کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6320522 | 1619720 | 2016 | 8 صفحه PDF | دانلود رایگان |
- Sample limitation is a common challenge to bioactivity screening studies.
- Low-volume methods were applied in androgen-receptor reporter gene cell-based tests.
- (Ant)agonism by five chemicals was measured with miniaturized and regular methods.
- Results were within assay acceptance criteria and in same range as regular tests.
- Downscaling procedures can contribute to future bioactivity screening studies.
Bioactivity screening studies often face sample amount limitation with respect to the need for reliable, reproducible and quantitative results. Therefore approaches that minimize sample use are needed. Low-volume exposure and chemical dilution procedures were applied in an androgen receptor reporter gene human cell line assay to evaluate environmental contaminants and androgen receptor modulators, which were the agonist 5α-dihydrotestosterone (DHT); and the antagonists flutamide, bisphenol A, 1-hydroxypyrene and triclosan. Cells were exposed in around 1/3 of the medium volume recommended by the protocol (70 μL/well). Further, chemical losses during pipetting steps were minimized by applying a low-volume method for compound dilution in medium (250 μL for triplicate wells) inside microvolume glass inserts. Simultaneously, compounds were evaluated following conventional procedures (200 μL/well, dilution in 24-well plates) for comparison of results. Low-volume exposure tests produced DHT EC50 (3.4-3.7 Ã 10â 10 M) and flutamide IC50 (2.2-3.3 Ã 10â 7 M) values very similar to those from regular assays (3.1-4.2 Ã 10â 10 and 2.1-3.3 Ã 10â 7 M respectively) and previous studies. Also, results were within assay acceptance criteria, supporting the relevance of the downscaling setup for agonistic and antagonistic tests. The low-volume exposure was also successful in determining IC50 values for 1-hydroxypyrene (2.1-2.8 Ã 10â 6 M), bisphenol A (2.6-3.3 Ã 10â 6 M), and triclosan (1.2-1.9 Ã 10â 6 M) in agreement with values obtained through high-volume exposure (2.3-2.8, 2.5-3.4 and 1.0-1.3 Ã 10â 6 M respectively). Finally, experiments following both low-volume dosing and exposure produced flutamide and triclosan IC50 values similar to those from regular tests. The low-volume experimental procedures provide a simple and effective solution for studies that need to minimize bioassay sample use while maintaining method reliability. The downscaling methods can be applied for the evaluation of samples, fractions or chemicals which require minimal losses during the steps of pipetting, transference to medium and exposure in bioassays.
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Journal: Science of The Total Environment - Volume 571, 15 November 2016, Pages 826-833