کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6326197 | 1619751 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ecotoxicological assessment of soil microbial community tolerance to glyphosate
ترجمه فارسی عنوان
ارزیابی اکولوژیک سم شناسی تحمل جامعه میکروبی خاک به گلیفوزات
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DGGEIC50UPGMACLPPPICTq-PCR - Q-PCRSoil microbial community - جامعه میکروبی خاکquantitative real time PCR - زمان واقعی PCR کمیRespiratory quotient - سهم تنفسیactive ingredient - ماده فعالCommunity level physiological profiling - مشخصات فیزیولوژیکی سطح جامعهhalf maximal inhibitory concentration - نیمه حداکثر غلظت مهاریGlyphosate - گلیفوزات
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
چکیده انگلیسی
Glyphosate is the most used herbicide worldwide. While contrasting results have been observed related with its impact on soil microbial communities, more studies are necessary to elucidate the potential effects of the herbicide. Differences in tolerance detected by Pollution Induced Community Tolerance (PICT) approach could reflect these effects. The objective of the present study was to assess the tolerance to glyphosate (the active ingredient and a commercial formulation) of contrasting soils with (H) and without (NH) history of exposure. The hypothesis of a higher tolerance in H soils due to a sustained selection pressure on community structure was tested through the PICT approach. Results indicated that tolerance to glyphosate is not consistent with previous history of exposure to the herbicide either for the active ingredient or for a commercial formulation. Soils of H and NH sites were also characterized in order to determine to what extent they differ in their functional diversity and structure of microbial communities. Denaturant Gradient Gel Electrophoresis (DGGE) and Quantitative Real Time PCR (Q-PCR) indicated high similarity of Eubacteria profiles as well as no significant differences in abundance, respectively, between H and NH sites. Community level physiological profiling (CLPP) indicated some differences in respiration of specific sources but functional diversity was very similar as reflected by catabolic evenness (E). These results support PICT assay, which ideally requires soils with differences in their exposure to the contaminant but minor differences in other characteristics. This is, to our knowledge, the first report of PICT approach with glyphosate examining tolerance at soil microbial community level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science of The Total Environment - Volume 533, 15 November 2015, Pages 60-68
Journal: Science of The Total Environment - Volume 533, 15 November 2015, Pages 60-68
نویسندگان
Marco Allegrini, MarÃa Celina Zabaloy, Elena del V. Gómez,