کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6332615 1619794 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of rainfall events on the occurrence and detection efficiency of viruses in river water impacted by combined sewer overflows
ترجمه فارسی عنوان
اثرات حوادث بارندگی بر وقوع و بازدهی تشخیص ویروس ها در آب های رودخانه تحت تأثیر جریان های فاضلاب تلفیقی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
چکیده انگلیسی
Rainfall events can introduce large amount of microbial contaminants including human enteric viruses into surface water by intermittent discharges from combined sewer overflows (CSOs). The present study aimed to investigate the effect of rainfall events on viral loads in surface waters impacted by CSO and the reliability of molecular methods for detection of enteric viruses. The reliability of virus detection in the samples was assessed by using process controls for virus concentration, nucleic acid extraction and reverse transcription (RT)-quantitative PCR (qPCR) steps, which allowed accurate estimation of virus detection efficiencies. Recovery efficiencies of poliovirus in river water samples collected during rainfall events (< 10%) were lower than those during dry weather conditions (> 10%). The log10-transformed virus concentration efficiency was negatively correlated with suspended solid concentration (r2 = 0.86) that increased significantly during rainfall events. Efficiencies of DNA extraction and qPCR steps determined with adenovirus type 5 and a primer sharing control, respectively, were lower in dry weather. However, no clear relationship was observed between organic water quality parameters and efficiencies of these two steps. Observed concentrations of indigenous enteric adenoviruses, GII-noroviruses, enteroviruses, and Aichi viruses increased during rainfall events even though the virus concentration efficiency was presumed to be lower than in dry weather. The present study highlights the importance of using appropriate process controls to evaluate accurately the concentration of water borne enteric viruses in natural waters impacted by wastewater discharge, stormwater, and CSOs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science of The Total Environment - Volumes 468–469, 15 January 2014, Pages 757-763
نویسندگان
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