Determination of low urinary 8-hydroxy-2`-deoxyguanosine excretion with capillary electrophoresis and molecularly imprinted monolith solid phase microextraction

► 8-Hydroxy-2`-deoxyguanosine (8-OHdG) is a sensitive biomarker of DNA oxidative damage. ► Urinary 8-OHdG determination is confounded by trace content and complex matrix. ► High extraction efficiency and selectivity were exhibited for the prepared monolith used as extraction sorbent. ► Coupled to capillary electrophoresis, a precise approach was developed for urinary 8-OHdG determination. ► 106 subjects with relative low 8-OHdG excretions were analyzed successfully.