Real-time PCR detection of Ochroconis lascauxensis involved in the formation of black stains in the Lascaux Cave, France

► A real-time PCR assay was designed to detect Ochroconis lascauxensis. ► The fungus is the principal causal agent of the black stains in Lascaux Cave, France. ► The data confirmed the wide dissemination of the fungus in all cave compartments.