Earthworm bioassays and seedling emergence for monitoring toxicity, aging and bioaccumulation of anthropogenic waste indicator compounds in biosolids-amended soil

► Biosolids toxicity was measured by earthworm bioassays and seed germination. ► Earthworm survival and reproduction are sensitive to biosolids at agronomic rates. ► Biosolids toxicity to earthworms increased with increased biosolids aging. ► Bioavailability of some organic contaminants increased with biosolids aging. ► Seed germination is sensitive to agronomic application rates of biosolids.