کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
634639 | 1456071 | 2013 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Human hepatic cell behavior on polysulfone membrane with double porosity level Human hepatic cell behavior on polysulfone membrane with double porosity level](/preview/png/634639.png)
In the membrane-based bioartificial livers developed up to now, the hepatic cells were located either in the fibers′ lumen or in the cartridge, with limited capacity for cell hosting. Here, we designed a polysulfone (PSU) membrane with a double porosity level: (i) surface macroporosity emerging in macrochambers accessible to hepatic cell colonization; (ii) microporosity to ensure gas and molecule transfers between macrochambers and supernatant, as well as potential immune barrier. ESEM and X-ray tomography confirmed that macrochambers accessed the membrane surface and were interconnected. Biocompatibility and performances of this PSU membrane with double porosity level were compared to classical semi-permeable structures, following cell organization, cell proliferation and liver specific activities over a 9 days incubation. Macrochambers were colonized by hepatic cells, leading to higher albumin synthesis compared to control. Therefore, this membrane with double porosity appeared as a promising support to offer an inner 3D environment adequate to cell proliferation to form a liver-like tissue.
► We designed a polysulfone membrane characterized by a double (micro/macro) porosity level.
► Tomo-X and ESEM analysis showed an original inner structure of the membrane.
► Hepatic cell behavior and functionality confirmed the membrane biocompatibility.
Journal: Journal of Membrane Science - Volume 428, 1 February 2013, Pages 454–461