Impairment of uterine smooth muscle contractions and prostaglandin secretion from cattle myometrium and corpus luteum in vitro is influenced by DDT, DDE and HCH

- DDT, DDE and HCH stimulated bovine myometrial contractions.
- The studied xenobiotics affect prostaglandin secretion from bovine myometrium and CL.
- DDT and DDE increased the mRNA expression of LIF, which can stimulate PG production.
- Prostaglandins are involved in adverse effect of xenobiotics on uterine contractions.
- DDT, DDE and HCH can reduce reproductive success.

The aim of this study was to investigate the effect of dichlorodiphenyltrichloroethane(DDT), dichlorodiphenyldichloroethylene (DDE) and γ-hexachlorocyclohexane (HCH) (10 ng/ml) on myometrial motility and the secretory function of the myometrium and corpus luteum (CL) collected from cows on days 8-12 of the estrous cycle. All of the xenobiotics increased (P<0.05) myometrial contractility. Moreover, the xenobiotics stimulated the secretion of the following prostaglandins (PGs) from myometrial strips: PGF2α, PGE2 and PGI2. DDT and DDE also increased (P<0.05) the release of PGF2α from CL strips, and HCH had the same effect (P<0.05) on the secretion of PGE2 and PGI2. The studied xenobiotics did not affect (P>0.05) PG synthesis, but DDT and DDE increased the mRNA expression levels of leukemia inhibitor factor (LIF), which can stimulate PG production. In summary, the xenobiotics affected PG secretion from cow myometrium and CL, which may contribute to the mechanism of uterine contraction disturbance.