Characterization of nanoporous membranes for implementation in an osmotic glucose sensor based on the concanavalin A-dextran affinity assay

► Nanoporous membranes exhibit permeation rates for molecules larger than rated MWCO. ► Membrane architecture plays as an important factor as pore size with respect to MWCO. ► Alumina membranes (4-6 nm pores) exhibit best properties with respect to rated MWCO. ► The viscous matrix of the affinity assay prevents the efflux of assay components. ► Non-ideal osmotic behaviour increase specific sensitivity in the measurement range.