کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
636775 | 1456131 | 2010 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Utilizing transmembrane convection to enhance solute sampling and delivery by microdialysis: Theory and in vitro validation
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کلمات کلیدی
CEDPESMWCOaCSFFLRUltrafiltration - اولترافیلتراسیونConvection-enhanced delivery - تحویل تحویل ConvectionFluorescein - فلورسئینMolecular weight cutoff - قطع وزن مولکولیartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیMathematical modeling - مدل سازی ریاضیmicrodialysis - میکرو دیالیزConvection - همرفتDiffusion - واپخش یا انتشار Molecular weight - وزن مولکولیPES, Polyethersulfone - پلی اتر سولفون
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
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چکیده انگلیسی
Microdialysis is a well-developed membrane-based tool relying on diffusion to sample diffusible constituents of complex media, such as biological tissue. The objective of this research is to expand the utility of microdialysis by combining transmembrane convection with diffusion to enhance solute exchange between microdialysis probes and the surrounding medium. We have developed a mathematical model to describe probe performance and performed validation experiments utilizing tracer solutes and commercially available probes with 100-kDa molecular weight cutoff membranes. Diffusive and fluid permeabilities of the probe membranes are evaluated for probes immersed in well-stirred bathing media in vitro. Transmembrane convection alters the solute extraction fraction, i.e., the fractional loss of a solute from the probe perfusate during delivery and the fractional gain by the perfusate during sampling. The extraction fraction change depends upon the magnitude and direction (inward or outward) of fluid movement across the membrane. However, for solutes with zero reflection coefficients, equality is maintained between these delivery and sampling extraction fractions. This equality is a prerequisite for probe calibration approaches that rely on analyte delivery from the perfusate. Thus, we have provided the theoretical and experimental basis for exploiting convection in a quantitative manner to enhance solute delivery and sampling in microdialysis applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Membrane Science - Volume 348, Issues 1â2, 15 February 2010, Pages 131-149
Journal: Journal of Membrane Science - Volume 348, Issues 1â2, 15 February 2010, Pages 131-149
نویسندگان
Peter M. Bungay, Tianli Wang, Hua Yang, William F. Elmquist,