کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
637047 | 1456139 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Permeation of blood cells from umbilical cord blood through surface-modified polyurethane foaming membranes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Blood cell permeation of umbilical cord blood (UCB) through surface-modified foamed polyurethane membranes having carboxylic group (PU-COOH) with different pore sizes (i.e., 5, 8.9 and 12 μm) was investigated. Red blood cells and platelets can permeate through PU-COOH membranes with any pore sizes analyzed, while leukocytes and hematopoietic stem and progenitor cells (HSPCs) cannot permeate through PU-COOH membranes with permeation ratio of less than 5%. Several recovery solutions (e.g., 20% dextran, 0.5 wt% serum albumin solution, plasma solution) were evaluated for the isolation of several blood cells including HSPCs from PU-COOH membranes after UCB permeated through the membranes. Recovery ratios of leukocytes were relatively low through PU-COOH membranes having pore size (r) = 5 μm, while higher recovery ratio of RBCs was observed than that of leukocytes. The recovery ratio of HSPCs was found to be 8-27% depending on the membrane pore sizes and recovery solution. The recovery ratio of HSPCs from UCB (8-27% in this study) was found to be less than that from peripheral blood reported previously (60-80% [A. Higuchi, M. Sekiya, Y. Gomei, M. Sakurai, W.-Y. Chen, S. Egashira, Y. Matsuoka, Separation of hematopoietic stem cells from human peripheral blood through modified polyurethane foaming membranes, J. Biomed. Mater. Res.: A 85A (4) (2008) 853-861]). This suggests that the purification of HSPCs form UCB is significantly difficult compared to that from peripheral blood, because of high expression of adhesion proteins of HSPCs in UCB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Membrane Science - Volume 339, Issues 1â2, 1 September 2009, Pages 184-188
Journal: Journal of Membrane Science - Volume 339, Issues 1â2, 1 September 2009, Pages 184-188
نویسندگان
Akon Higuchi, Siou-Ting Yang, Pei-Tsz Li, Ruoh-chyu Ruaan, Wen-Yih Chen, Yung Chang, Yu Chang, Eing Mei Tsai, Yung Hung Chen, Han-Chow Wang, Shih-Tien Hsu, Qing-Dong Ling,