کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6373053 | 1624296 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Monitoring changes in the actinobacterial field communities present in the rhizosphere soil of a transgenic cotton producing Cry1Ab/Ac proteins
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Monitoring changes in the actinobacterial field communities present in the rhizosphere soil of a transgenic cotton producing Cry1Ab/Ac proteins Monitoring changes in the actinobacterial field communities present in the rhizosphere soil of a transgenic cotton producing Cry1Ab/Ac proteins](/preview/png/6373053.png)
چکیده انگلیسی
Transgenic cotton GK 12 producing Cry1Ab/Ac proteins can effectively control lepidopteran pests in agriculture. However, there are concerns that GK 12 has unintended side-effects on actinobacterial communities in the soil. Actinobacterial communities in the rhizosphere soil of cottons were analyzed by real-time PCR (qPCR) and denaturing gradient gel electrophoresis (DGGE) at different growth stages in three annual replications under field conditions. Population sizes and community structures of Actinobacteria were markedly affected by natural variations in years, growth stages and cultivars. No significant differences were found in population sizes (excepting at budding in 2009), community structures, and Shannon indices of Actinobacteria (excepting at budding in 2010) between the GK 12 and its non-transgenic parent Simian 3, although there were differences between the GK 12 and the conventional cotton DP 5415. Phylogenetic analysis of DGGE band sequences indicated that Cellulosimicrobium sp. and Rhodococcus sp. only occurred in the GK 12 and the Simian 3. The dominant Actinobacteria did not differ between the GK 12 and the Simian 3. In conclusion, the overall impacts of the GK 12 on actinobacterial communities in the rhizosphere soil, based on qPCR and DGGE, were not significant during three continuous years in northern China.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Crop Protection - Volume 91, January 2017, Pages 1-7
Journal: Crop Protection - Volume 91, January 2017, Pages 1-7
نویسندگان
Yan-Jun Zhang, Ming Xie, Qian Li, Xiao-Lin Zhang, Zhao-Rong Zhang,