Characterization of lignin derived from water-only flowthrough pretreatment of Miscanthus

- Lignin solubilization plays an important role in flowthrough pretreatment process.
- Flowthrough pretreatment of Miscanthus biomass led to lignin solubilization.
- Lignin solubilization was associated with preferential removal of S-lignin.
- Pretreated Miscanthus biomass documented significant reduction of S/G ratio.
- Bond cleavage within lignin polymer was revealed during flowthrough pretreatment.