Apigenin di-C-glycosides (ACG) content and composition in grains of bread wheat (Triticum aestivum) and related species

► Genotype controlled the major portion of the variation. ► ACG content appeared to be a multigenic trait. ► Variation in ACG1/ACG2 was associated with a limited number of chromosomes. ► The ACG ratio provides understanding on the genetic regulation of C-glycosylation. ► It is possible to manipulate ACG content and composition traits through breeding.